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Article

Cloning, sequencing, and expression of Bacillus subtilis genes involved in ATP-dependent nuclease synthesis.

Journal of bacteriology - 1 Jun 1991

Kooistra J, Venema G

Abstract excerpt

The genes encoding the subunits of the Bacillus subtilis ATP-dependent nuclease (add genes) have been cloned. The genes were located on an 8.8-kb SalI-SmaI chromosomal DNA fragment. Transformants of a recBCD deletion mutant of Escherichia coli with plasmid pGV1 carrying this DNA fragment showed ATP-dependent nuclease activity. Three open reading frames were identified on the 8.8-kb SalI-SmaI fragment, which could...

Topics

Amino Acid Sequence
Bacillus subtilis
Base Sequence
DNA Topoisomerases, Type I
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Escherichia coli Proteins
Exodeoxyribonuclease V
Exodeoxyribonucleases
Gene Expression Regulation, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Bacterial

Molecular Sequence Data

Mutation

Phenotype

Plasmids

Promoter Regions, Genetic

Restriction Mapping

Sequence Alignment

Source

Open in PubMed Open full text

Identifiers

PMID: 1646786
DOI: 10.1128/jb.173.12.3644-3655.1991
PMCID: PMC207991

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1646786-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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