Article
Identification of four unique clones encoding 10 kDa proteins from Bacillus that cause phenotypic complementation of a phoA mutant strain of Escherichia coli.
Journal of general microbiology - 1 Mar 1991
Lee J W, Edwards C W, Hulett F M
Abstract excerpt
A number of clones have been isolated from two Bacillus species which complement the PhoA- phenotype of Escherichia coli mutants under conditions that induce the expression of alkaline phosphatase (APase). These clones were initially thought to carry XPases because the transformed host could hydr...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Bacillus
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Humans
- Molecular Sequence Data
- Open Reading Frames
- Phenotype
- Promoter Regions, Genetic
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Transformation, Bacterial