Article
Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene.
Journal of bacteriology - 1 Apr 1990
Cardin R D, Biel A J
Abstract excerpt
We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2.85-kilobase EcoRI-PstI fragment.
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