Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene.

doi:10.1128/jb.172.4.2181-2183.1990

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Article

Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene.

Journal of bacteriology - 1 Apr 1990

Cardin R D, Biel A J

Abstract excerpt

We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2...

Topics

Bacteriochlorophylls
Cloning, Molecular
Cosmids
DNA Transposable Elements
Escherichia coli
Genes, Bacterial
Kinetics
Mutation
Rhodopseudomonas

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