Article
Isolation of poly-3-hydroxybutyrate metabolism genes from complex microbial communities by phenotypic complementation of bacterial mutants.
Applied and environmental microbiology - 1 Jan 2006
Wang Chunxia, Meek David J, Panchal Priya, Boruvka Natalie, Archibald Frederick S, Driscoll Brian T, Charles Trevor C
Abstract excerpt
The goal of this study was to initiate investigation of the genetics of bacterial poly-3-hydroxybutyrate (PHB) metabolism at the community level. We constructed metagenome libraries from activated sludge and soil microbial communities in the broad-host-range IncP cosmid pRK7813. Several unique clones were isolated from these libraries by functional heterologous complementation of a Sinorhizobium meliloti bdhA...
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