Article
Transcriptional silencing induced by Arabidopsis T-DNA mutants is associated with 35S promoter siRNAs and requires genes involved in siRNA-mediated chromatin silencing.
The Plant journal : for cell and molecular biology - 1 Nov 2010
Mlotshwa Sizolwenkosi, Pruss Gail J, Gao Zhihuan, Mgutshini Nomathamsanqa L, Li Junjie, Chen Xuemei, Bowman Lewis H, Vance Vicki
Abstract excerpt
The utility of many T-DNA insertion mutant lines of Arabidopsis is compromised by their propensity to trigger transcriptional silencing of transgenes expressed from the CaMV 35S promoter. To try to circumvent this problem, we characterized the genetic requirements for maintenance of 35S promoter homology-dependent transcriptional gene silencing induced by the dcl3-1 (SALK_005512) T-DNA insertion mutant line....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.