Article
[In-vitro amplification of oval cells with preservation of stem cell phenotype].
Zhonghua bing li xue za zhi = Chinese journal of pathology - 1 Aug 2010
Chen Qiong-rong, Liu Fang, Zhao Guo-qiang, Xue Ling, Hu Rui-de, Wu Hui-xi, Zhang Meng
Abstract excerpt
OBJECTIVE: To explore cell culture techniques for amplification of oval cells with preservation simultaneously of the stem cell characteristics. METHODS: Oval cell line OC3 was cultured in RPMI 1640 supplemented with 15% fetal bovine serum and 20 µg/L EGF. Cells were harvested every 5 passages an...
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