Article
2-Hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine induction of recombinational mutations in mammalian cell lines as detected by DNA fingerprinting.
Molecular carcinogenesis - 1 Feb 1994
Kitazawa T, Kominami R, Tanaka R, Wakabayashi K, Nagao M
Abstract excerpt
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant mutagenic heterocyclic amine in cooked foods. Two mouse tumor cell lines, BMT11 and FM3A, were exposed to its proximate form, 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP). Fifty-six subclones of BMT11 and 39 subclones of FM3A were isolated and analyzed by DNA fingerprinting. Treatment with 10-20 microM N-OH-PhIP gave...
Topics
- Animals
- Base Sequence
- Clone Cells
- DNA Damage
- DNA Fingerprinting
- DNA, Neoplasm
- Imidazoles
- Mammary Neoplasms, Experimental
- Mice
- Mice, Inbred C3H
- Mice, Inbred C57BL