Article
Nonsense suppression in Dictyostelium discoideum.
Developmental genetics - 1 Jan 1990
Dingermann T, Reindl N, Brechner T, Werner H, Nerke K
Abstract excerpt
We describe the generation of Dictyostelium discoideum cell lines that carry different suppressor tRNA genes. These genes were constructed by primer-directed mutagenesis changing a tRNA(Trp)(CCA) gene from D. discoideum to a tRNA(Trp)(amber) gene and changing a tRNA(Glu)(UUC) gene from D. discoideum to a tRNA(Glu)(ochre) as well as a tRNA(Glu)(amber) gene. These genes were stably integrated into the D. discoideum...
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