Article
Gene function analysis by amber stop codon suppression: CMBF is a nuclear protein that supports growth and development of Dictyostelium amoebae.
Journal of molecular biology - 26 Jan 2001
Winckler T, Trautwein C, Tschepke C, Neuhäuser C, Zündorf I, Beck P, Vogel G, Dingermann T
Abstract excerpt
The C-module-binding factor, CMBF, is a nuclear DNA-binding protein which was originally identified through its specific binding to a promoter element within the retrotransposable element TRE5-A of Dictyostelium discoideum AX2 cells. In order to analyse putative physiological functions of CMBF for the TRE5-A-hosting D. discoideum cells, we used a novel strategy to create mutant cell lines which stably...
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