Article
A single base exchange in an intron of the Dictyostelium discoideum alpha-actinin gene inhibits correct splicing of the RNA but allows transport to the cytoplasm and translation.
The Journal of biological chemistry - 5 Jan 1990
Witke W, Noegel A A
Abstract excerpt
The genomic structure of the alpha-actinin gene from Dictyostelium discoideum has been determined. The coding region consists of three exons separated by two short introns located at either end of the gene. In an alpha-actinin mutant, HG1130, about 1% of alpha-actinin protein products are found compared to the parent strain AX2. The size of the mutant proteins are 88 and 95 kDa. In HG1130, the second intron is...
Topics
- Actinin
- Base Sequence
- Cytoplasm
- DNA Probes
- DNA Restriction Enzymes
- Dictyostelium
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Polymerase Chain Reaction