Article
Intrinsic fluorescence of succinyl-CoA synthetase and four tryptophan mutants. Tryptophan 76 and tryptophan 248 of the beta-subunit are responsive to CoA binding.
Biochemistry - 30 Jan 1990
Nishimura J S, Mann C J, Ybarra J, Mitchell T, Horowitz P M
Abstract excerpt
Previous studies showed that modification of an average of one of the three tryptophan residues of succinyl-CoA synthetase of Escherichia coli abolished enzyme activity, but did not prevent phosphorylation of the enzyme by ATP [Ybarra, J., Prasad, A. R. S., & Nishimura, J.S. (1986) Biochemistry 2...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphate
- Coenzyme A
- Coenzyme A Ligases
- Iodides
- Mutation
- Phenylalanine
- Protein Binding
- Spectrometry, Fluorescence
- Succinate-CoA Ligases
- Succinates
- Tryptophan