Article
[PCR in combination with silver as a method for the determination of apolipoprotein E alleles].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 10 Oct 1998
Zhang A, Han Z, Wang L, Tao G
Abstract excerpt
OBJECTIVE: To present a rapid, sensitive and safe procedure for the determination of human apolipoprotein E alleles. METHODS: Genomic DNA was extracted from 0.5ml whole blood by a non-phenol protocol. After PCR and restriction enzyme digestion,short DNA fragments were detected by a simplified sil...
Topics
- Alleles
- Alzheimer Disease
- Apolipoproteins E
- Coloring Agents
- DNA
- Humans
- Polymerase Chain Reaction
- Silver
