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Associate PCR-RFLP assay design with SNPs based on genetic algorithm in appropriate parameters estimation.

IEEE transactions on nanobioscience - 1 Jun 2013

Chuang Li-Yeh, Cheng Yu-Huei, Yang Cheng-Huei, Yang Cheng-Hong

Abstract excerpt

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is a commonly used laboratory technique and useful in small-scale basic research studies of complex genetic diseases that are associated with single nucleotide polymorphisms (SNPs). Before PCR-RFLP assay for SNP genotyping can be performed, a feasible primer pair observes numerous constraints and an available restriction enzyme for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 23722280
DOI: 10.1109/TNB.2013.2258469

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