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Application of real-time PCR and melting curve analysis in rapid Diego blood group genotyping.

Immunohematology - 1 Jan 2010

Novaretti M C Z, Ruiz A S, Dorlhiac-Llacer P E, Chamone D A F

Abstract excerpt

The paucity of appropriate reagents for serologic typing of the Diego blood group antigens has prompted the development of a real-time PCR and melting curve analysis for Diego blood group genotyping. In this study, we phenotyped 4326 donor blood samples for Di(a) using semiautomated equipment. All 157 Di(a+) samples were then genotyped by PCR using sequence-specific primers (PCR-SSP) for DI*02 because of...

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PMID: 20932077

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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