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Article

Development of Real-time PCR and Melting Curve Analysis for the Rapid Detection of DEL with RHD (c.1222T>C) or RHD (c.1227G>A).

Clinical laboratory - 1 Oct 2016

Hong Yun Ji, Kim Hyungsuk, Song Eun Young, Cho Duck, Park Kyoung Un, Han Kyou-Sup

Abstract excerpt

BACKGROUND: DEL, a variant of RhD, is difficult to detect in routine blood bank testing owing to its extremely low levels of D antigen expression. However, DEL is capable of alloimmunizing a patient when transfused into RhDnegative individuals. METHODS: In this study, we developed real-time PCR and melting curve analysis for the rapid detection of the DEL phenotype. RESULTS: Of the 325 serologically RhD-negative...

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Source

Identifiers

PMID: 28164536
DOI: 10.7754/Clin.Lab.2016.160320

Genes

RHD

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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