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Article

A novel set of real-time PCRs for rapid differentiation between human cytomegalovirus wild-type and highly passaged laboratory strains.

Journal of virological methods - 1 Dec 2010

Ziemann Malte, Unmack Alexander, Hennig Holger

Abstract excerpt

Restricting amplification of human cytomegalovirus (HCMV) DNA to wild-type (WT) HCMV is useful to exclude PCR contaminations by laboratory strains from viral cultures. A set of UL141-specific TaqMan PCRs was developed to amplify (1) WT HCMV and laboratory strain Towne(long), but not strain AD169, (2) only WT HCMV. The performance was compared to a PCR targeting the conserved sequence of HCMV glycoprotein B using...

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PMID: 20887751
DOI: 10.1016/j.jviromet.2010.09.020

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