Article
Comparative evaluation of published cytomegalovirus primers for rapid real-time PCR: which are the most sensitive?
11 Jun 2009
Abstract excerpt
Standardization of human cytomegalovirus (CMV) PCR is highly recommended. As primer design is essential for PCR sensitivity, this study evaluated all published CMV primer pairs to identify the most sensitive for single-round real-time PCR. PubMed (1993-2004) was searched for original papers aimed at CMV PCR. Fifty-seven papers were identified revealing 82 different primer pairs. Of these, 17 primer sets were...
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