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Article

Caffeine and paraxanthine HPLC assay for CYP1A2 phenotype assessment using saliva and plasma.

Biomedical chromatography : BMC - 1 Oct 2010

Perera Vidya, Gross Annette S, McLachlan Andrew J

Abstract excerpt

Caffeine has been extensively used as a probe to measure CYP1A2 activity in humans with caffeine clearance or the paraxanthine (major metabolite of caffeine) to caffeine concentration ratio being regarded as the preferred metric. A simple reverse-phased C(18) HPLC assay using ethyl acetate liquid-liquid extraction was developed to quantitate caffeine and paraxanthine concentrations in saliva and plasma. The...

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Identifiers

PMID: 20853468
DOI: 10.1002/bmc.1419

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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