Article
A semi-nested real-time PCR method to detect low chimerism percentage in small quantity of hematopoietic stem cell transplant DNA samples.
Genome - 1 Feb 2017
Aloisio Michelangelo, Bortot Barbara, Gandin Ilaria, Severini Giovanni Maria, Athanasakis Emmanouil
Abstract excerpt
Chimerism status evaluation of post-allogeneic hematopoietic stem cell transplantation samples is essential to predict post-transplant relapse. The most commonly used technique capable of detecting small increments of chimerism is quantitative real-time PCR. Although this method is already used in several laboratories, previously described protocols often lack sensitivity and the amount of the DNA required for...
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