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Article

Stimulation and suppression of PCR-mediated recombination

1 Apr 1998

Abstract excerpt

Recombination, or chimera formation, is known to occur between related template sequences present in a single PCR amplification. To characterize the conditions under which such recombinant amplification products form we monitored the exchange of sequence between two homologous templates carrying different restriction sites separated by 282 bp. Using a typical cycling program the rates of recombination between the...

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Identifiers

PMID: 9512558
DOI: 10.1093/nar/26.7.1819

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