Article
Differential modulation of growth and phenotypic expression of chondrocytes in sparse and confluent cultures by growth factors in cartilage.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Oct 1990
Hiraki Y, Inoue H, Asada A, Suzuki F
Abstract excerpt
The growth-promoting actions of cartilage extracts (CE) on rabbit cultured chondrocytes were studied to assess the role of local acting growth factors in the generation and expansion of highly differentiated cells. In the present study, DNA synthesis and proteoglycan synthesis in the cultured chondrocytes were monitored by flow cytofluorometry and double-isotope autoradiography by using [3H]thymidine and...
Topics
- Animals
- Autoradiography
- Cartilage
- Cell Division
- Cells, Cultured
- DNA
- Flow Cytometry
- Growth Substances
- Male
- Phenotype
- Proteoglycans