Article
Modulation of sulfated proteoglycan synthesis and collagen gene expression by chondrocytes grown in the presence of bFGF alone or combined with IGF1.
Reproduction, nutrition, development - 1 Jan 1990
Nataf V, Tsagris L, Dumontier M F, Bonaventure J, Corvol M
Abstract excerpt
Prepubertal rabbit epiphyseal chondrocytes were grown in high density primary culture for 3 d. They were then incubated for 3 additional d in serum-free culture medium to which bFGF (1-50 ng/ml) was added. During the last 24 h incubation period, either IGF1 (1-80 ng/ml) or Insulin (1-5 micrograms...
Topics
- Animals
- Cell Differentiation
- Cell Division
- Cells, Cultured
- Collagen
- DNA Probes
- Fibroblast Growth Factors
- Gene Expression
- Growth Plate
- Insulin
- Insulin-Like Growth Factor I
- Nucleic Acid Hybridization
- Phenotype
- Proteoglycans
- RNA, Messenger
- Rabbits
- Somatomedins
- Sulfates