Article
Establishment of a growth hormone responsive chondrogenic cell line from fetal rat tibia.
Molecular and cellular endocrinology - 1 Feb 1993
Ohlsson C, Nilsson A, Swolin D, Isaksson O G, Lindahl A
Abstract excerpt
Reproducible effects of growth hormone (GH) on primary isolated cells in monolayer are highly dependent on the culture conditions and/or the fraction of GH responsive cells. To study the effect of GH at the cellular level, a homogenous cell line with both GH responsiveness and chondrogenic properties was established. Primary isolated cells from 18-day-old fetal rat tibia were subcultured using a strict protocol...
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