Article
Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1: enzymes and catalysis.
The FEBS journal - 1 Oct 2010
Hayashi Keiko, Yasuda Kaori, Sugimoto Hiroshi, Ikushiro Shinichi, Kamakura Masaki, Kittaka Atsushi, Horst Ronald L, Chen Tai C, Ohta Miho, Shiro Yoshitsugu, Sakaki Toshiyuki
Abstract excerpt
Our previous studies revealed that the double variant of cytochrome P450 (CYP)105A1, R73V/R84A, has a high ability to convert vitamin D(3) to its biologically active form, 1α,25-dihydroxyvitamin D(3) [1α,25(OH)(2)D(3)], suggesting the possibility for R73V/R84A to produce 1α,25(OH)(2)D(3). Because Actinomycetes, including Streptomyces, exhibit properties that have potential advantages in the synthesis of secondary...
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