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Article

Protein engineering of CYP105s for their industrial uses.

Biochimica et biophysica acta. Proteins and proteomics - 1 Jan 2018

Yasuda Kaori, Sugimoto Hiroshi, Hayashi Keiko, Takita Teisuke, Yasukawa Kiyoshi, Ohta Miho, Kamakura Masaki, Ikushiro Shinichi, Shiro Yoshitsugu, Sakaki Toshiyuki

Abstract excerpt

Cytochrome P450 enzymes belonging to the CYP105 family are predominantly found in bacteria belonging to the phylum Actinobacteria and the order Actinomycetales. In this review, we focused on the protein engineering of P450s belonging to the CYP105 family for industrial use. Two Arg substitutions to Ala of CYP105A1 enhanced its vitamin D3 25- and 1α-hydroxylation activities by 400 and 100-fold, respectively. The...

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PMID: 28583351
DOI: 10.1016/j.bbapap.2017.05.014

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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