Article
The NMR solution structure of subunit G (G(61)(-)(101)) of the eukaryotic V1VO ATPase from Saccharomyces cerevisiae.
Biochimica et biophysica acta - 1 Oct 2010
Rishikesan Sankaranarayanan, Manimekalai Malathy S S, Grüber Gerhard
Abstract excerpt
Subunit G is an essential stalk subunit of the eukaryotic proton pump V(1)V(O) ATPase. Previously the structure of the N-terminal region, G(1)(-)(59), of the 13kDa subunit G was solved at higher resolution. Here solution NMR was performed to determine the structure of the recombinant C-terminal region (G(61)(-)(101)) of subunit G of the Saccharomyces cerevisiae V(1)V(O) ATPase. The protein forms an extended...
Topics
- Amino Acid Sequence
- Circular Dichroism
- Cysteine
- Electrophoresis, Polyacrylamide Gel
- Eukaryotic Cells
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Multimerization