Article
Mutational analysis of subunit G (Vma10p) of the yeast vacuolar H+-ATPase.
The Journal of biological chemistry - 24 Nov 2000
Charsky C M, Schumann N J, Kane P M
Abstract excerpt
The G subunit of V-ATPases is a soluble subunit that shows homology with the b subunit of F-ATPases and may be part of the "stator" stalk connecting the peripheral V(1) and membrane V(0) sectors. When the N-terminal half of the G subunit is modeled as an alpha helix, most of the conserved residues fall on one face of the helix (Hunt, I. E., and Bowman, B. J. (1997) J. Bioenerg. Biomembr. 29, 533-540). We probed...
Topics
- Amino Acid Sequence
- Conserved Sequence
- DNA Mutational Analysis
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Protein Conformation
- Protein Structure, Secondary
- Proton-Translocating ATPases
- Structure-Activity Relationship
- Vacuolar Proton-Translocating ATPases
- Yeasts