Article
Fluorescence analysis of tryptophan-containing variants of the LamB signal sequence upon insertion into a lipid bilayer.
Biochemistry - 25 Jun 1991
McKnight C J, Rafalski M, Gierasch L M
Abstract excerpt
To investigate the interaction of the LamB signal sequence with lipid bilayers, we have synthesized three tryptophan-containing analogues of the wild-type signal peptide. The tryptophan residues were used as intrinsic fluorescent probes of the N-terminal (position 5), central (position 18), and C-terminal (position 24) regions of the 25-residue peptide. The tryptophan substitutions did not significantly alter the...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Escherichia coli
- Fluorescent Dyes
- Genetic Variation
- Lipid Bilayers
- Molecular Sequence Data
- Porins
- Protein Conformation
- Protein Engineering
- Protein Sorting Signals