Article
Conformational changes of FtsZ reported by tryptophan mutants.
Biochemistry - 31 May 2011
Chen Yaodong, Erickson Harold P
Abstract excerpt
E. coli FtsZ has no native tryptophan. We showed previously that the mutant FtsZ L68W gave a 2.5-fold increase in trp fluorescence when assembly was induced by GTP. L68 is probably buried in the protofilament interface upon assembly, causing the fluorescence increase. In the present study we introduced trp residues at several other locations and examined them for assembly-induced fluorescence changes. L189W,...
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