Article
The role of N53Q mutation on the rat mu-opioid receptor function.
Journal of biomolecular techniques : JBT - 1 Jul 2010
Rostami A, Rabbani M, Mir-Mohammad-Sadeghi M
Abstract excerpt
Glycosylation of the mu-opioid receptor may play an important role on its function. Using nested PCR, N53Q mutation was prepared in the N-terminal region of the rat mu-opioid receptor cDNA and cloned into the pcDNA3 vector. The plasmids containing the wild-type and mutated receptor cDNA were transfected into the COS-7 cells. Intracellular cAMP was measured in the morphine-treated and untreated transfected cells...
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