Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Method for designing and optimizing random-search libraries for strain improvement.

Applied and environmental microbiology - 1 Aug 2010

Klein-Marcuschamer Daniel, Stephanopoulos Gregory

Abstract excerpt

Random searches have been the hallmark of directed evolution and have been extensively employed in the improvement of complex or poorly understood phenotypes such as tolerance to toxic compounds in the context of cellular engineering. While genome-wide mutagenesis followed by selection or screeni...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Directed Molecular Evolution
Share to Genetic Engineering
Share to Genetic Testing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genomics

Share to Industrial Microbiology

Share to Mutation

Source

Open in PubMed Open full text

Identifiers

PMID: 20581192
DOI: 10.1128/AEM.00828-10
PMCID: PMC2918980

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-20581192-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Method for designing and optimizing random-search libraries for strain improvement.PMID 20581192Read ↗

Combinatorial engineering of microbes for optimizing cellular phenotype.Semantic proximity Read ↗

Tools for genome-wide strain design and construction.Semantic proximity Read ↗

Assessing the potential of mutational strategies to elicit new phenotypes in industrial strains.Semantic proximity Read ↗

Random mutagenesis libraries: optimization and simplification by PCR.Semantic proximity Read ↗

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production.Semantic proximity Read ↗

The evolving art of creating genetic diversity: From directed evolution to synthetic biology.Semantic proximity Read ↗

Strain improvement of industrially important microorganisms based on resistance to toxic metabolites and abiotic stress.Semantic proximity Read ↗

[Progress in the construction and screening of random mutation library].Semantic proximity Read ↗

Random mutagenesis using a mutator strain.Semantic proximity Read ↗

Model-guided mechanism discovery and parameter selection for directed evolution.Semantic proximity Read ↗

Molecular evolution of a defined DNA sequence with accumulation of mutations in a single round by a dual approach to random chemical mutagenesis (DuARCheM).Semantic proximity Read ↗

Pooled CRISPR screening enables genotype-phenotype association in industrial microorganisms.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.