Article
Carbon-13 NMR study of switch variant anti-dansyl antibodies: antigen binding and domain-domain interactions.
Biochemistry - 2 Jul 1991
Kato K, Matsunaga C, Odaka A, Yamato S, Takaha W, Shimada I, Arata Y
Abstract excerpt
A 13C NMR study is reported of switch variant anti-dansyl antibodies, which possess the identical VH, VL, and CL domains in conjunction with highly homologous but not identical heavy-chain constant regions. Each of these antibodies has been selectively labeled with 13C at the carbonyl carbon of T...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites, Antibody
- Carbon Isotopes
- Cell Line
- Dansyl Compounds
- Genetic Variation
- Immunoglobulin Fab Fragments
- Immunoglobulin Fc Fragments
- Immunoglobulin G
- Immunoglobulin Switch Region
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Molecular Sequence Data
- Protein Conformation