Article
Carbon-13 NMR study of switch variant anti-dansyl antibodies: antigen binding and domain-domain interactions.
Biochemistry - 2 Jul 1991
Kato K, Matsunaga C, Odaka A, Yamato S, Takaha W, Shimada I, Arata Y
Abstract excerpt
A 13C NMR study is reported of switch variant anti-dansyl antibodies, which possess the identical VH, VL, and CL domains in conjunction with highly homologous but not identical heavy-chain constant regions. Each of these antibodies has been selectively labeled with 13C at the carbonyl carbon of Trp, Tyr, His, or Cys residue by growing hybridoma cells in serum-free medium. Spectral assignments have been made by...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.