Article
A multinuclear NMR study of the affinity maturation of anti-NP mouse monoclonal antibodies: comparison of antibody combining sites of primary response antibody N1G9 and secondary response antibody 3B62.
Biochemistry - 21 Dec 1993
Nakayama T, Arata Y, Shimada I
Abstract excerpt
On the basis of multinuclear NMR data, the structures of the antibody combining sites of anti-4-hydroxy-3-nitrophenylacetyl (NP) antibodies were compared for N1G9, which is one of the primary response antibodies with low affinity for NP, and 3B62, which is one of the secondary response antibodies...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Affinity
- Binding Sites, Antibody
- Cell Line
- Immunoglobulin Fab Fragments
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutation
- Nitrophenols
- Phenylacetates
- Sequence Homology, Amino Acid