Article
Isolation of cell wall mutants in Aspergillus nidulans by screening for hypersensitivity to Calcofluor White.
Mycologia - 1 Jan 2000
Hill Terry W, Loprete Darlene M, Momany Michelle, Ha Youngsil, Harsch Lisa M, Livesay Jennifer A, Mirchandani Amit, Murdock Jeremy J, Vaughan Michael J, Watt Mridula B
Abstract excerpt
As a first step toward identifying novel genes of wall metabolism in filamentous fungi, we have screened a collection of Aspergillus nidulans mutants for strains exhibiting hypersensitivity toward the chitin binding agent Calcofluor White (CFW). This strategy has been used previously to identify cell wall mutants in Saccharomyces cerevisiae. We have identified 10 mutants representing eight loci, designated calA...
Topics
- Aspergillus nidulans
- Benzenesulfonates
- Cell Wall
- Culture Media
- Fluorescent Dyes
- Microbial Sensitivity Tests
- Mutation
- Osmolar Concentration
- Phenotype
- Temperature