Article
Cloning and functional analysis of the ubiquitin-specific protease gene UBP1 of Saccharomyces cerevisiae.
The Journal of biological chemistry - 25 Jun 1991
Tobias J W, Varshavsky A
Abstract excerpt
In eukaryotes, both natural and engineered fusions of ubiquitin to itself or other proteins are cleaved by processing proteases after the last (Gly76) residue of ubiquitin. Using the method of sib selection, and taking advantage of the fact that bacteria such as Escherichia coli lack ubiquitin-specific enzymes, we have cloned a gene, named UBP1, of the yeast Saccharomyces cerevisiae that encodes a...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA, Fungal
- Endopeptidases
- Molecular Sequence Data
- Mutation
- Plasmids
- Saccharomyces cerevisiae