Activity of artificial mutant variants of human growth hormone deficient in a disulfide bond between Cys53 and Cys165.

doi:10.1248/cpb.39.150

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Article

Activity of artificial mutant variants of human growth hormone deficient in a disulfide bond between Cys53 and Cys165.

Chemical & pharmaceutical bulletin - 1 Jan 1991

Uchida E, Uemura H, Tanaka T, Nishikawa S, Uesugi S, Tanaka A, Morikawa M, Hayakawa T, Ikehara M

Abstract excerpt

In order to understand the role of Cys53 and Cys165 of human growth hormone (hGH) in receptor-binding and biological activity, artificial mutant variants of hGH were prepared in Escherichia coli by in vitro mutagenesis. Variants of hGH were constructed by replacement of Cys165 with Ala ([Ala165]h...

Topics

Animals
Base Sequence
Cystine
Disulfides
Growth Hormone
Humans
Molecular Sequence Data
Mutation
Protein Conformation

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