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Article

Inhibition of thrombin formation by active site mutated (S360A) activated protein C.

The Journal of biological chemistry - 23 Jul 2010

Nicolaes Gerry A F, Bock Paul E, Segers Kenneth, Wildhagen Karin C A A, Dahlbäck Björn, Rosing Jan

Abstract excerpt

Activated protein C (APC) down-regulates thrombin formation through proteolytic inactivation of factor Va (FVa) by cleavage at Arg(506) and Arg(306) and of factor VIIIa (FVIIIa) by cleavage at Arg(336) and Arg(562). To study substrate recognition by APC, active site-mutated APC (APC(S360A)) was used, which lacks proteolytic activity but exhibits anticoagulant activity. Experiments in model systems and in plasma...

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Source

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Identifiers

PMID: 20484050
DOI: 10.1074/jbc.M110.131029
PMCID: PMC2906281

Genes

APC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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Inhibition of thrombin formation by active site mutated (S360A) activated protein C.PMID 20484050Read ↗

Factor Va is inactivated by activated protein C in the absence of cleavage sites at Arg-306, Arg-506, and Arg-679.Semantic proximity Read ↗

Role of P1 residues Arg336 and Arg562 in the activated-Protein-C-catalysed inactivation of Factor VIIIa.Semantic proximity Read ↗

Effects of factor Xa and protein S on the individual activated protein C-mediated cleavages of coagulation factor Va.Semantic proximity Read ↗

Contribution of factor VIII light-chain residues 2007-2016 to an activated protein C-interactive site.Semantic proximity Read ↗

The structure-function relationship of activated protein C. Lessons from natural and engineered mutations.Semantic proximity Read ↗

Identification of surface epitopes of human coagulation factor Va that are important for interaction with activated protein C and heparin.Semantic proximity Read ↗

Effects of prothrombin on the individual activated protein C-mediated cleavages of coagulation factor Va.Semantic proximity Read ↗

Activated factor X cleaves factor VIII at arginine 562, limiting its cofactor efficiency.Semantic proximity Read ↗

Detailed mechanisms of the inactivation of factor VIIIa by activated protein C in the presence of its cofactors, protein S and factor V.Semantic proximity Read ↗

Molecular characterization of an extended binding site for coagulation factor Va in the positive exosite of activated protein C.Semantic proximity Read ↗

Novel APC-cleavage sites in FVa provide insights into mechanisms of action of APC and its cofactor protein S.Semantic proximity Read ↗

Basic residues in the 37-loop of activated protein C modulate inhibition by protein C inhibitor but not by alpha(1)-antitrypsin.Semantic proximity Read ↗

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