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Article

Optimized protocol for detection of HIV-1 drug mutations in patients with low viral load.

Journal of virological methods - 1 Sept 2010

Stelzl Evelyn, Troppan Katharina T, Winkler Michaela, Korn Klaus, Kessler Harald H

Abstract excerpt

The TRUGENE HIV-1 Genotyping Kit for HIV-1 drug resistance testing is limited by the need for samples with HIV-1 RNA loads of at least 1000 copies/mL. In order to enable sequencing of clinical samples with viral loads under 1000 copies/mL, an optimized automated sample preparation protocol on the VERSANT kPCR Sample Preparation (SP) Module was evaluated. In order to prove the concept of successful sequencing of...

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Identifiers

PMID: 20471425
DOI: 10.1016/j.jviromet.2010.05.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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