Article
siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis.
Nucleic acids research - 1 Sept 2010
Yu Bin, Bi Liu, Zhai Jixian, Agarwal Manu, Li Shengben, Wu Qingfa, Ding Shou-Wei, Meyers Blake C, Vaucheret Hervé, Chen Xuemei
Abstract excerpt
Plant microRNAs (miRNAs) and small interfering RNAs (siRNAs) bear a 2'-O-methyl group on the 3'-terminal nucleotide. This methyl group is post-synthetically added by the methyltransferase protein HEN1 and protects small RNAs from enzymatic activities that target the 3'-OH. A mutagenesis screen fo...
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