Decoupling mutations in the D-channel of the aa(3)-type cytochrome c oxidase from Rhodobacter sphaeroides suggest that a continuous hydrogen-bonded chain of waters is essential for proton pumping.

Abstract excerpt

The aa(3)-type cytochrome c oxidase from Rhodobacter sphaeroides utilizes two proton-input channels to provide all the protons for chemistry (water formation) and proton pumping. The D-channel is responsible for the uptake of all pumped protons, four protons per O(2). Several substitutions of either N139 or N207, near the entrance of the D-channel, were previously reported to decouple the proton pump from oxidase...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Binding Sites
• Share to Electron Transport Complex IV