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Article

High-throughput beta-thalassemia carrier screening by allele-specific Q-primer real-time polymerase chain reaction.

Analytical biochemistry - 1 Sept 2010

Liu Xiaokun, Law Hai Yang, Tan Yuen Ming, Hong Yan

Abstract excerpt

Based on a novel Q-primer real-time polymerase chain reaction (PCR) system, we designed allele-specific Q-primers for the detection of three beta-thalassemia mutations [Cd41/42(-TCTT), IVSI nt5 (G>C), and IVSII nt654 (C>T)] that have a high carrier frequency in Southeast Asia. With clear distinction between heterozygote and wild-type, DeltaC(t) (threshold cycle) values were defined. The results of evaluating 139...

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Identifiers

PMID: 20433808
DOI: 10.1016/j.ab.2010.04.025

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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