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Article

Application of the Single-Molecule Real-Time Technology (SMRT) for Identification of HKαα Thalassemia Allele.

Laboratory medicine - 5 Jan 2023

Zhang Min, Lin Zhaodong, Chen Meihuan, Pan Yali, Zhang Yanhong, Chen Lingji, Lin Na, Ren Yuanyuan, Jia Hongjin, Cai Meiying, Xu Liangpu, Huang Hailong

Abstract excerpt

OBJECTIVE: Single-molecule real-time technology (SMRT) is a sequencing technology using the DNA polymerases and fluorescently tagged nucleotides to accurately sequence DNA strands. The purpose of this study was to evaluate the detection accuracy of SMRT for identification of the Hong Kongαα (HKαα) thalassemia allele. METHODS: We conducted a blinded study of 33 samples of known HKαα alleles. These alleles were...

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PMID: 36053226
DOI: 10.1093/labmed/lmac065
PMCID: PMC9825284

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