Article
Enhanced fluorescent properties of an OmpT site deleted mutant of green fluorescent protein.
Microbial cell factories - 29 Apr 2010
Salunkhe Shardul S, Raiker Veena A, Rewanwar Sachin, Kotwal Prakash, Kumar Avijeet, Padmanabhan Sriram
Abstract excerpt
BACKGROUND: The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. Expression of recombinant GFP in E. coli K12 host from pBAD24M-GFP construct upon arabinose induction was significantly lower than that seen in E. coli B cells with higher expression at 30 degrees C as compared...
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