Article
High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis I.
Cell cycle (Georgetown, Tex.) - 1 May 2010
Rumpf Cornelia, Cipak Lubos, Novatchkova Maria, Li Zhang, Polakova Silvia, Dudas Andrej, Kovacikova Ines, Miadokova Eva, Ammerer Gustav, Gregan Juraj
Abstract excerpt
Meiosis is the process which produces haploid gametes from diploid precursor cells. This reduction of chromosome number is achieved by two successive divisions. Whereas homologs segregate during meiosis I, sister chromatids segregate during meiosis II. To identify novel proteins required for proper segregation of chromosomes during meiosis, we applied a high-throughput knockout technique to delete 87 S. pombe...
Topics
- Cell Cycle Proteins
- Chromosome Segregation
- Chromosomes, Fungal
- Dyneins
- Gene Knockout Techniques
- Meiosis
- Phenotype
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Up-Regulation