Article
Monitoring genomic alterations with a panel of oligonucleotide probes specific for various simple repeat motifs.
Electrophoresis - 1 Jan 2000
Nürnberg P, Barth I, Fuhrmann E, Lenzner C, Losanova T, Peters C, Pöche H, Thiel G
Abstract excerpt
Germline and somatic instability of the human genome was studied, using synthetic oligonucleotides specific for simple repeat motifs. The following probes were used: (GTG)5, (GACA)4, (GATA)4, (CT)8, (TTAGGG)3, (GT)8, (GAA)6 and (GGAT)4. Each of them is unique with respect to the target regions recognized in the genome. Thus compilation of the various fingerprint data provides a complex map of the genome (and its...
Topics
- Base Sequence
- DNA Fingerprinting
- Female
- Glioma
- Humans
- Lymphocytes
- Male
- Mutation
- Oligonucleotide Probes
- Pedigree
- Repetitive Sequences, Nucleic Acid