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Article

Spectral [corrected] studies on the cadmium-ion-binding properties of bovine brain S-100b protein.

The Biochemical journal - 15 May 1991

Donato H, Mani R S, Kay C M

Abstract excerpt

The effect of Cd2+ binding on bovine brain S-100b protein was studied using c.d. u.v. difference spectroscopy and fluorescence measurements. At pH 7.5, S-100b protein binds two Cd2+ ions per monomer with a Kd value of 3 x 10(-5) M. Addition of Cd2+ resulted in perturbing the single tyrosine residue (Tyr17) in the protein as indicated by u.v. difference spectroscopy and aromatic c.d. measurements. In the presence...

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Identifiers

PMID: 2039467
DOI: 10.1042/bj2760013
PMCID: PMC1151136

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