Determination of DNA binding specificities of mutated zinc finger domains.

doi:10.1016/0014-5793(91)80545-e

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Article

Determination of DNA binding specificities of mutated zinc finger domains.

FEBS letters - 20 May 1991

Thiesen H J, Bach C

Abstract excerpt

By substituting non conserved amino acids present in the postulated alpha-helical region of zinc finger domains, we demonstrated that Cys2/His2 type zinc finger domains could be targeted to new DNA binding sites. The putative alpha-helical region of the second SP1 zinc finger (RSDELQRH) was repla...

Topics

Base Sequence
Chimera
Chromatography, Liquid
DNA
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression Regulation, Bacterial
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Zinc Fingers

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