Article
Monitoring of NY-ESO-1 specific CD4+ T cells using molecularly defined MHC class II/His-tag-peptide tetramers.
Proceedings of the National Academy of Sciences of the United States of America - 20 Apr 2010
Ayyoub Maha, Dojcinovic Danijel, Pignon Pascale, Raimbaud Isabelle, Schmidt Julien, Luescher Immanuel, Valmori Danila
Abstract excerpt
MHC-peptide tetramers have become essential tools for T-cell analysis, but few MHC class II tetramers incorporating peptides from human tumor and self-antigens have been developed. Among limiting factors are the high polymorphism of class II molecules and the low binding capacity of the peptides. Here, we report the generation of molecularly defined tetramers using His-tagged peptides and isolation of folded...
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