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Article

Maintenance and killing efficiency of conditional lethal constructs in Pseudomonas putida.

Journal of industrial microbiology - 1 Aug 1992

Bej A K, Molin S, Perlin M, Atlas R M

Abstract excerpt

Conditional lethal (suicidal) genetic constructs were designed and employed in strains of Pseudomonads as models for containment of genetically-engineered microbes that may be deliberately released into the environment. A strain of Pseudomonas putida was formed with a suicide vector designated pBAP24h that was constructed by cloning the host killing gene (hok) into the RSF1010 plasmid pVDtac24 and placing it...

Topics

Cloning, Molecular
Containment of Biohazards
Genes, Bacterial
Genes, Lethal
Kinetics
Phenotype
Pseudomonas putida

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 1368479
DOI: 10.1007/BF01583839

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1368479-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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