Article
Maintenance and killing efficiency of conditional lethal constructs in Pseudomonas putida.
Journal of industrial microbiology - 1 Aug 1992
Bej A K, Molin S, Perlin M, Atlas R M
Abstract excerpt
Conditional lethal (suicidal) genetic constructs were designed and employed in strains of Pseudomonads as models for containment of genetically-engineered microbes that may be deliberately released into the environment. A strain of Pseudomonas putida was formed with a suicide vector designated pBAP24h that was constructed by cloning the host killing gene (hok) into the RSF1010 plasmid pVDtac24 and placing it...
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