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Article

Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase .

Biochemistry - 4 May 2010

Jorns Marilyn Schuman, Chen Zhi-Wei, Mathews F Scott

Abstract excerpt

Oxygen reduction and sarcosine oxidation in monomeric sarcosine oxidase (MSOX) occur at separate sites above the si- and re-faces, respectively, of the flavin ring. Mutagenesis studies implicate Lys265 as the oxygen activation site. Substitution of Lys265 with a neutral (Met, Gln, or Ala) or basic (Arg) residue results in an approximately 10(4)- or 250-fold decrease, respectively, in the reaction rate. The...

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Identifiers

PMID: 20353187
DOI: 10.1021/bi100160j
PMCID: PMC2912214

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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